The presence of extracellular microRNAs in the media of cultured Drosophila cells.
Sci Rep
; 8(1): 17312, 2018 11 23.
Article
en En
| MEDLINE
| ID: mdl-30470777
ABSTRACT
While regulatory RNA pathways, such as RNAi, have commonly been described at an intracellular level, studies investigating extracellular RNA species in insects are lacking. In the present study, we demonstrate the presence of extracellular microRNAs (miRNAs) in the cell-free conditioned media of two Drosophila cell lines. More specifically, by means of quantitative real-time PCR (qRT-PCR), we analysed the presence of twelve miRNAs in extracellular vesicles (EVs) and in extracellular Argonaute-1 containing immunoprecipitates, obtained from the cell-free conditioned media of S2 and Cl.8 cell cultures. Next-generation RNA-sequencing data confirmed our qRT-PCR results and provided evidence for selective miRNA secretion in EVs. To our knowledge, this is the first time that miRNAs have been identified in the extracellular medium of cultured cells derived from insects, the most speciose group of animals.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Medios de Cultivo Condicionados
/
Proteínas de Drosophila
/
MicroARNs
/
Drosophila melanogaster
/
Proteínas Argonautas
/
Vesículas Extracelulares
Límite:
Animals
Idioma:
En
Revista:
Sci Rep
Año:
2018
Tipo del documento:
Article
País de afiliación:
Bélgica