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A smartphone-based on-site nucleic acid testing platform at point-of-care settings.
Xu, Xiayu; Wang, Xuemin; Hu, Jie; Gong, Yang; Wang, Lin; Zhou, Wan; Li, XiuJun; Xu, Feng.
Afiliación
  • Xu X; The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Shaanxi, P. R. China.
  • Wang X; Bioinspired Engineering & Biomechanics Center (BEBC), Xi'an Jiaotong University, Shaanxi, P. R. China.
  • Hu J; The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Shaanxi, P. R. China.
  • Gong Y; Bioinspired Engineering & Biomechanics Center (BEBC), Xi'an Jiaotong University, Shaanxi, P. R. China.
  • Wang L; The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Shaanxi, P. R. China.
  • Zhou W; Bioinspired Engineering & Biomechanics Center (BEBC), Xi'an Jiaotong University, Shaanxi, P. R. China.
  • Li X; The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Shaanxi, P. R. China.
  • Xu F; Bioinspired Engineering & Biomechanics Center (BEBC), Xi'an Jiaotong University, Shaanxi, P. R. China.
Electrophoresis ; 40(6): 914-921, 2019 03.
Article en En | MEDLINE | ID: mdl-30511768
ABSTRACT
We developed a smartphone-based on-site nucleic acid testing (NAT) platform that can image and analyze lateral flow nucleic acid assays at point-of-care settings. An inexpensive add-on was devised to run lateral flow assays while providing homogeneous ambient light for imaging. In addition, an Android app with a user-friendly interface was developed for the result analysis and management. Linear color calibration is implemented inside the app to minimize the colorimetric reaction difference between smartphones. A relationship function between nucleic acid concentration and colorimetric reaction was established and evaluated by leave-one-out cross validation. The predicted concentration and true concentration showed a good agreement with an R-squared value of 0.96. This smartphone-based NAT platform can be used to diagnose infectious diseases and monitor disease progression, and assess treatment efficacy, especially for resource-limited settings.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Sistemas de Atención de Punto / Técnicas de Amplificación de Ácido Nucleico / Aplicaciones Móviles / Teléfono Inteligente Idioma: En Revista: Electrophoresis Año: 2019 Tipo del documento: Article
Texto completo
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PubMed Links
Buscar en Google

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Sistemas de Atención de Punto / Técnicas de Amplificación de Ácido Nucleico / Aplicaciones Móviles / Teléfono Inteligente Idioma: En Revista: Electrophoresis Año: 2019 Tipo del documento: Article