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A Chemogenetic Approach for the Optical Monitoring of Voltage in Neurons.
Sundukova, Mayya; Prifti, Efthymia; Bucci, Annalisa; Kirillova, Kseniia; Serrao, Joana; Reymond, Luc; Umebayashi, Miwa; Hovius, Ruud; Riezman, Howard; Johnsson, Kai; Heppenstall, Paul A.
Afiliación
  • Sundukova M; Molecular Medicine Partnership Unit (MMPU), 69117, Heidelberg, Germany.
  • Prifti E; Epigenetics and Neurobiology Unit, EMBL Rome, via Ramarini 32, Monterotondo, Italy.
  • Bucci A; Molecular Medicine Partnership Unit (MMPU), 69117, Heidelberg, Germany.
  • Kirillova K; Epigenetics and Neurobiology Unit, EMBL Rome, via Ramarini 32, Monterotondo, Italy.
  • Serrao J; Molecular Medicine Partnership Unit (MMPU), 69117, Heidelberg, Germany.
  • Reymond L; Epigenetics and Neurobiology Unit, EMBL Rome, via Ramarini 32, Monterotondo, Italy.
  • Umebayashi M; Molecular Medicine Partnership Unit (MMPU), 69117, Heidelberg, Germany.
  • Hovius R; Epigenetics and Neurobiology Unit, EMBL Rome, via Ramarini 32, Monterotondo, Italy.
  • Riezman H; Molecular Medicine Partnership Unit (MMPU), 69117, Heidelberg, Germany.
  • Johnsson K; Epigenetics and Neurobiology Unit, EMBL Rome, via Ramarini 32, Monterotondo, Italy.
  • Heppenstall PA; Ecole Polytechnique Federale de Lausanne, ISIC, National Centre for Competence in Research (NCCR) in Chemical Biology, 1015, Lausanne, Switzerland.
Angew Chem Int Ed Engl ; 58(8): 2341-2344, 2019 02 18.
Article en En | MEDLINE | ID: mdl-30569539
ABSTRACT
Optical monitoring of neuronal voltage using fluorescent indicators is a powerful approach for the interrogation of the cellular and molecular logic of the nervous system. Herein, a semisynthetic tethered voltage indicator (STeVI1) based upon nile red is described that displays voltage sensitivity when genetically targeted to neuronal membranes. This environmentally sensitive probe allows for wash-free imaging and faithfully detects supra- and sub-threshold activity in neurons.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Oxazinas / Imagen Óptica / Colorantes Fluorescentes / Neuronas Límite: Humans Idioma: En Revista: Angew Chem Int Ed Engl Año: 2019 Tipo del documento: Article País de afiliación: Alemania
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Oxazinas / Imagen Óptica / Colorantes Fluorescentes / Neuronas Límite: Humans Idioma: En Revista: Angew Chem Int Ed Engl Año: 2019 Tipo del documento: Article País de afiliación: Alemania