UPLC-MS/MS method for the quantification of ertugliflozin and sitagliptin in rat plasma.
Anal Biochem
; 567: 112-116, 2019 02 15.
Article
en En
| MEDLINE
| ID: mdl-30578763
ABSTRACT
In the present study, an ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) approach was designed to concurrently measure the levels of ertugliflozin and sitagliptin in rat plasma with diazepam as the internal standard (IS). Acetonitrile-based protein precipitation was applied for sample preparation, then analytes (ertugliflozin and sitagliptin) were subjected to gradient elution chromatography with a mobile phase composed of acetonitrile (A) and 0.1% formic acid in water (B). Ertugliflozin was monitored by m/z 437.2â¯ââ¯329.0 transition for quantification and m/z 437.2â¯ââ¯207.5 transition for qualification, and sitagliptin was determined by m/z 408.2â¯ââ¯235.0 transition for quantification and m/z 408.2â¯ââ¯174.0 transition for qualification by multiple reaction monitoring (MRM) in positive ion electrospray ionization (ESI) source. When the concentration of ertugliflozin ranged from 1 to 1000â¯ng/mL and sitagliptin ranged from 2 to 2500â¯ng/mL, the method exhibited good linearity. For both ertugliflozin and sitagliptin, the intra- and inter-day precision were determined with the values of 1.6-10.9% and 0.8-13.3%, respectively; and the accuracy ranged from -5.7% to 14.6%. Matrix effect, extraction recovery, and stability data were in line with the stipulated FDA guidelines for validating a bioanalytical method. The validity of the designed method was confirmed through the pharmacokinetic experiments.
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1
Bases de datos:
MEDLINE
Asunto principal:
Compuestos Bicíclicos Heterocíclicos con Puentes
/
Fosfato de Sitagliptina
Límite:
Animals
Idioma:
En
Revista:
Anal Biochem
Año:
2019
Tipo del documento:
Article