A novel SERS-based aptasensor for ultrasensitive sensing of microcystin-LR.
Food Chem
; 278: 197-202, 2019 Apr 25.
Article
en En
| MEDLINE
| ID: mdl-30583362
ABSTRACT
We developed a novel aptasensor based on surface enhanced Raman spectroscopy (SERS) and applied it for highly sensitive detection of microcystin-LR (MC-LR). In this work, MC-LR aptamer and its corresponding complementary DNA fragments (cDNA) were conjugated to gold nanoparticles (AuNPs) and magnetic nanoparticles (MNPs), respectively. Then, MC-LR aptamer-AuNPs and cDNA-MNPs conjugates were used as signal probes and capture probes, respectively. The proposed assay exhibited a linearity range from 0.01 to 200â¯ng/mL with the limit of detection (LOD) of 0.002â¯ng/mL. In addition, the reliability of the novel approach was validated by detecting different levels of MC-LR spiked in tap water samples. Overall, the novel aptasensor paves a new way for rapid and accurate determination of MC-LR and can be referred to detect other hazardous substances in water products.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Espectrometría Raman
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Técnicas Biosensibles
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Aptámeros de Nucleótidos
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Microcistinas
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Límite de Detección
Idioma:
En
Revista:
Food Chem
Año:
2019
Tipo del documento:
Article
País de afiliación:
China