[Effect of Modified Leweiyin Recipe on SGC-7901 Human Gastric Cancer Cells from Crosstalk between NF-κB and STAT3].
Zhongguo Zhong Xi Yi Jie He Za Zhi
; 37(2): 232-236, 2017 Feb.
Article
en Zh
| MEDLINE
| ID: mdl-30650279
ABSTRACT
Objective To observe the effect of Modified Leweiyin Recipe (MLR) on nuclear factor kappa B (NF-κB) and crosstalk between signal transducers and activators of transcription 3 (STAT3) in SGC-7901 human gastric cancer cells. Methods SGC-7901 human gastric cancer cell strain was taken as subjects. The vectors of NF-κB (ReIA/p65)-PECFP and STAT3-PEYFP were constructed and transfected in SGC-7901 human gastric cancer cells. Cells were then intervened by MLR after stimulated by LPS. The crosstalk between NF-κB and STAT3 in cells was detected using fluorescence resonance energy transfer and co-immunoprecipitation. Results After LPS stimulation, the crosstalk between NF-κB and STAT3 was enhanced. But it was significantly weakened after MLR intervention. Conclusion MLR could treat precancerous lesions of gastric cancer and prevent the occurrence of gastric cancer possibly by blocking the crosstalk between NF-κB and STAT3.
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Bases de datos:
MEDLINE
Asunto principal:
Neoplasias Gástricas
/
Medicamentos Herbarios Chinos
/
FN-kappa B
/
Factor de Transcripción STAT3
Límite:
Humans
Idioma:
Zh
Revista:
Zhongguo Zhong Xi Yi Jie He Za Zhi
Asunto de la revista:
TERAPIAS COMPLEMENTARES
Año:
2017
Tipo del documento:
Article