Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells.
J Vis Exp
; (143)2019 01 07.
Article
en En
| MEDLINE
| ID: mdl-30663671
ABSTRACT
Within the liver, lymphatic vessels are found within the portal triad, and their described function is to remove interstitial fluid from the liver to the lymph nodes where cellular debris and antigens can be surveyed. We are very interested in understanding how the lymphatic vasculature might be involved in inflammation and immune cell function within the liver. However, very little has been published establishing digestion protocols for the isolation of lymphatic endothelial cells (LECs) from the liver or specific markers that can be used to evaluate liver LECs on a per cell basis. Therefore, we optimized a method for the digestion and staining of the liver in order to evaluate the LEC population in the liver. We are confident that the method outlined here will be useful for the identification and isolation of LECs from the liver and will strengthen our understanding of how LECs respond to the liver microenvironment.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Biomarcadores
/
Células Endoteliales
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Vasos Linfáticos
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Citometría de Flujo
/
Hígado
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Vis Exp
Año:
2019
Tipo del documento:
Article