Effect of 177Lu-iPSMA on viability and DNA damage of human glioma cells subjected to hypoxia-mimetic conditions.
Appl Radiat Isot
; 146: 24-28, 2019 Apr.
Article
en En
| MEDLINE
| ID: mdl-30743222
ABSTRACT
The therapeutic potential of 177Lu-iPSMA on hypoxic cancer cells has not been yet demonstrated. The aim of this work was to evaluate the radiation dose effect of 177Lu-iPSMA on viability and DNA damage in U87MG human glioma cells subjected to hypoxia-mimetic conditions. U87MG cells treated with 177Lu-iPSMA were incubated with CoCl2 in order to induce hypoxia-mimetic conditions. The cytotoxic and genotoxic effect was evaluated with an in vitro viability test and a neutral comet assay. 177Lu-iPSMA decreased the cell viability and induced DNA double strand breaks in U87MG human glioma cells under hypoxia-mimetic conditions. 177Lu-iPSMA produced the maximum effect at 48â¯h, suggesting that this radiopharmaceutical could be used as a strategy for the treatment of human glioma hypoxic cells.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Radioisótopos
/
Glutamato Carboxipeptidasa II
/
Glioma
/
Lutecio
Límite:
Humans
Idioma:
En
Revista:
Appl Radiat Isot
Asunto de la revista:
MEDICINA NUCLEAR
/
SAUDE AMBIENTAL
Año:
2019
Tipo del documento:
Article
País de afiliación:
México