Species-specificity in the interaction of IgE with FcεRI between humans and rats.
Pharmazie
; 74(1): 29-33, 2019 01 01.
Article
en En
| MEDLINE
| ID: mdl-30782247
ABSTRACT
The interaction of immunoglobulin E (IgE) to its high-affinity receptor (FcεRI) plays a key role in triggering allergic reactions. However, it is still controversial whether species specificity influences the binding ability between humans and rodents. Recombinant hFcεRIα / RBL-2H3 and hFcεRIα / MGC-803 cells were prepared and sensitized with hIgE/anti-hIgE antibody or DNP-IgE/DNP-BSA, respectively. Species-specificity was investigated using immuno-fluorescent analysis, ß-hexosaminidase release rate assay, intracellular calcium concentration assay and apoptosis assay, and the results showed that there is species-specificity in IgE/FcεRI in humans and rats, and no cross-recognition between IgE and FcεRI in two species. These results should provide the experimental evidence for further research on the pathogenesis and the drug development of allergic diseases.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Inmunoglobulina E
/
Receptores de IgE
/
Hipersensibilidad
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Pharmazie
Asunto de la revista:
FARMACIA
Año:
2019
Tipo del documento:
Article