Method for Determining Gelatinolytic Activity in Tissue: In Situ Gelatin Zymography.
Methods Mol Biol
; 1952: 193-199, 2019.
Article
en En
| MEDLINE
| ID: mdl-30825175
ABSTRACT
To explore the physiological or pathological roles of proteases, it is important to be able to detect and precisely localize them in a tissue, to differentiate between inactive and active forms, as well as to quantify and determine the nature of the enzyme that degrades a given substrate. Here we present an in situ gelatin zymography method that allows for a precise localization of active gelatin-degrading enzymes in a tissue section. In this method, dye-quenched gelatin is put on top of a tissue section. During an incubation period, active gelatinolytic enzymes will degrade the substrate and fluorescent signals are emitted from the locations of these enzymes.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Gelatinasas
/
Pruebas de Enzimas
/
Microscopía Fluorescente
/
Microtomía
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2019
Tipo del documento:
Article
País de afiliación:
Noruega