TLR-5 agonist Salmonella abortus equi flagellin FliC enhances FliC-gD-based DNA vaccination against equine herpesvirus 1 infection.
Arch Virol
; 164(5): 1371-1382, 2019 May.
Article
en En
| MEDLINE
| ID: mdl-30888564
ABSTRACT
Equine herpesvirus 1 (EHV-1) induces serious respiratory infections, viral abortion, neurological signs, and neonatal mortality in horses. Despite the use of vaccines, EHV-1 infection also causes a high annual economic burden to the equine industry. The poor immunogenicity of and protection conferred by EHV-1 vaccines are the major factors responsible for the spread of EHV-1 infection. The present study examined the immunogenicity of a novel DNA vaccine co-expressing FliC, a flagellin protein, in Salmonella abortus equi and the gD protein of EHV-1. Mice and horses were immunized intramuscularly with the vaccine, and mice were challenged with EHV-1. Immunofluorescence and western blotting revealed that FliC and gD can be efficiently expressed in cells. This novel vaccine significantly increased gD-specific antibody and interferon gamma (IFN-γ) levels in immunized mice and horses. Compared with controls, the viral load and morbidity were markedly reduced in FliC-gD-immunized mice after they were challenged with EHV-1. Furthermore, the immunogenicity of FliC-gD in a natural host was tested. Our results indicate that vaccinated mice and horses exhibit increased humoral and improved cellular immune responses.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Vacunas Virales
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Proteínas del Envoltorio Viral
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Herpesvirus Équido 1
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Infecciones por Herpesviridae
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Vacunas de ADN
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Flagelina
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Anticuerpos Antivirales
Límite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Arch Virol
Año:
2019
Tipo del documento:
Article
País de afiliación:
China