Total flavone of Abelmoschus manihot ameliorates Crohn's disease by regulating the NFκB and MAPK signaling pathways.
Int J Mol Med
; 44(1): 324-334, 2019 Jul.
Article
en En
| MEDLINE
| ID: mdl-31059072
Crohn's disease (CD) is a chronic relapsing form of inflammatory bowel disease, and its pathogenesis remains unknown. Total flavone of Abelmoschus manihot L. Medic (TFA), has been used as antiinflammatory and myocardial ischemia protective drug. The present study aimed to explore the effects of TFA on CD and its underlying mechanism. We reported that TFA comprises eight flavone glycosides, including quercetin3Orobinobioside, gossypetin3Oglucoside, quercetin3'Oglucoside, isoquercetin, hyperoside, myricetin, gossypetin and quercetin. In vivo, TFA promoted the survival of 2,4,6trinitrobenzene sulfonic acid (TNBS)induced colitis in mice, decreased weight loss and increased colon length in a dosedependent manner. Additionally, TFA notably ameliorated the inflammatory response in mice with TNBSinduced colitis as determined by histopathological analysis. In addition, the administration of TFA in mice with TNBSinduced colitis led to a significant decrease in the levels of cytokines in the sera and colon tissues; a significant decrease myeloperoxidase activity in the colon tissues was also observed. These findings may be associated with the suppression of the nuclear factorκB (NFκB) and mitogenactivated protein kinase (MAPK) signaling pathways. In vitro, TFA significantly downregulated the expression of cytokines in lipopolysaccharide (LPS)induced RAW264.7 cells. In addition, TFA suppressed LPSinduced activation of the NFκB and MAPK signaling pathways in RAW264.7 cells. Our findings indicated that TFA could suppress the inflammatory response in mice with TNBSinduced colitis via inhibition of the NFκB and MAPK signaling pathways. The results of the present study may improve understanding of the function of TFA and provide a novel theoretical basis for the treatment of CD.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Enfermedad de Crohn
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FN-kappa B
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Sistema de Señalización de MAP Quinasas
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Abelmoschus
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Flavonas
Límite:
Animals
Idioma:
En
Revista:
Int J Mol Med
Asunto de la revista:
BIOLOGIA MOLECULAR
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GENETICA MEDICA
Año:
2019
Tipo del documento:
Article