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Metabonomics of d-glucaro-1,4-lactone in preventing diethylnitrosamine-induced liver cancer in rats.
Yang, Wenlong; Zhou, Guanlin; Zou, Shubing; Yang, Wentao; Liu, Aihong; Sun, Shuilin; Xie, Baogang.
Afiliación
  • Yang W; a Department of Infectious Diseases , the Second Affiliated Hospital of Nanchang University; School of Pharmaceutical Science, Nanchang University , Nanchang , PR China.
  • Zhou G; a Department of Infectious Diseases , the Second Affiliated Hospital of Nanchang University; School of Pharmaceutical Science, Nanchang University , Nanchang , PR China.
  • Zou S; b Department of Hepatobiliary and Pancreatic Surgery , the Second Affiliated Hospital of Nanchang University , Nanchang , PR China.
  • Yang W; b Department of Hepatobiliary and Pancreatic Surgery , the Second Affiliated Hospital of Nanchang University , Nanchang , PR China.
  • Liu A; c Center of analysis and testing , Nanchang University , Nanchang , PR China.
  • Sun S; a Department of Infectious Diseases , the Second Affiliated Hospital of Nanchang University; School of Pharmaceutical Science, Nanchang University , Nanchang , PR China.
  • Xie B; a Department of Infectious Diseases , the Second Affiliated Hospital of Nanchang University; School of Pharmaceutical Science, Nanchang University , Nanchang , PR China.
Pharm Biol ; 56(1): 643-648, 2018 Dec.
Article en En | MEDLINE | ID: mdl-31070541
CONTEXT: d-Glucaro-1,4-lactone (1,4-GL) exists in many vegetables and fruits. Metabonomics has not been used to investigate the role of 1,4-GL in preventing liver cancer. OBJECTIVE: The pharmacological effects and metabolite alterations of 1,4-GL on the prevention of diethylnitrosamine (DEN)-induced liver cancer were investigated. MATERIALS AND METHODS: Ten healthy Sprague-Dawley rats served as control and 46 were used to establish rat liver cancer model. 1HNMR-based metabonomics was used to compare the effects of oral 1,4-GL (50 mg/kg) in liver cancer rats (n = 26) after 10 consecutive weeks of intervention. The amino acids in rat serum were quantified by HPLC-UV, and the changes in Fischer's ratio were calculated. RESULTS: The 20-week survival rate of DEN-induced liver cancer rats administered with oral 1,4-GL was increased from 45.0 to 70.0% with reduced carcinogenesis of the liver and significantly lowered serum α-fetoprotein level (14.28 ± 2.89 ng/mL vs. 18.56 ± 4.65 ng/mL, p = 0.012). The serum levels of leucine, valine, 3-hydroxybutyrate, lactate, acetate and glutamine in the DEN + 1,4-GL group returned to normal levels compared with those of the DEN group on week 20. Fischer's ratio in the rat serum of DEN group was 1.62 ± 0.21, which was significantly lower than that in healthy rats (2.3 ± 0.12). However, Fischer's ratio increased to 1.89 ± 0.22 in the DEN + 1,4-GL group. DISCUSSION AND CONCLUSIONS: 1,4-GL exerted positive effects on liver carcinogenesis in rats by pathological examination and metabonomic analysis. Its mechanism may be related to the restoration of amino acid and energy metabolism.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Dietilnitrosamina / Metabolómica / Ácido Glucárico / Neoplasias Hepáticas Experimentales Límite: Animals Idioma: En Revista: Pharm Biol Año: 2018 Tipo del documento: Article

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Dietilnitrosamina / Metabolómica / Ácido Glucárico / Neoplasias Hepáticas Experimentales Límite: Animals Idioma: En Revista: Pharm Biol Año: 2018 Tipo del documento: Article