Dynamic Radiolabeling of S-Palmitoylated Proteins.
Methods Mol Biol
; 2009: 111-127, 2019.
Article
en En
| MEDLINE
| ID: mdl-31152399
Proteins can be radiolabeled either during synthesis, typically using 35S-cysteine/methionine (35S-Cys/Met), or after synthesis, by adding a radiolabeled posttranslational modification. Here we describe how protein S-palmitoylation, and its dynamics, can be monitored by 3H-palmitate labeling and how the importance of S-palmitoylation in protein biogenesis and turnover can be investigated using 35S-Cys/Met pulse-chase metabolic labeling. Proteins frequently have multiple palmitoylation sites. The importance thereof on the design and interpretation of metabolic labeling experiments is discussed.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Radioisótopos de Azufre
/
Procesamiento Proteico-Postraduccional
/
Ácido Palmítico
/
Lipoilación
/
Marcaje Isotópico
Límite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2019
Tipo del documento:
Article
País de afiliación:
Suiza