Dynamic and structural insights into tick serpin from Ixodes ricinus.
J Biomol Struct Dyn
; 38(8): 2296-2303, 2020 May.
Article
en En
| MEDLINE
| ID: mdl-31215334
ABSTRACT
Ixodid ticks have a crucial impact on people and domestic animals worldwide. These parasites also pose a serious threat to livestock. To date, vaccination of hosts against ticks is a safer, more sustainable alternative to chemical control of ticks and the disease agents they transmit. Because of their roles in tick physiology, serpins (serine protease inhibitors) from tick saliva are among the candidates for anti-tick vaccines. Inhibitory serpins employ a suicide inhibition mechanism to inhibit proteases, where the serpin reactive centre loop (RCL) is cleaved, by the targeted protease, and then inserted into the main ß-sheet of the serpin. This causes a massive conformational change called the 'stressed to relaxed' (SâR) transition, leading to the breakdown of serpin into two regions (core domain and cleaved polypeptide). Recently, the first tick serpin crystal structure from Ixodes ricinus in R-state was reported. We thus employed molecular dynamics simulations to better understand serpin structure and dynamics in atomic detail. Overall, R-state serpin showed high rigidity, especially the core domain. The most flexible region is the terminal of the cleaved polypeptide, due to its high-water exposure, while the rest of the cleaved polypeptide is stably trapped behind the core domain. T363, D367 and N375 are found to play a vital role in protein-protein attachment. This finding can be used to explain the high stability of the R-state serpin at the atomic level and provides insight into this tick serpin which will be useful for rational anti-tick vaccine development. AbbreviationsMDMolecular DynamicsRCLReactive centre loopCommunicated by Ramaswamy H. Sarma.
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Bases de datos:
MEDLINE
Asunto principal:
Serpinas
/
Ixodes
Límite:
Animals
Idioma:
En
Revista:
J Biomol Struct Dyn
Año:
2020
Tipo del documento:
Article
País de afiliación:
Tailandia