Upregulated TTYH2 expression is critical for the invasion and migration of U2OS human osteosarcoma cell lines.
Biochem Biophys Res Commun
; 516(2): 521-525, 2019 08 20.
Article
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| MEDLINE
| ID: mdl-31230749
ABSTRACT
Ion channels have recently emerged as stable biomarkers and anticancer targets particularly when the applications of the currently available therapeutic regimens are limited, as in case of osteosarcoma, a malignant bone tumor. Here, we evaluated the expression of TTYH2, a presumably calcium-activated chloride channel, in a human osteosarcoma cell line U2OS. We used small-interfering RNA (siRNA)-mediated gene silencing to demonstrate the downregulation in the expression of TTYH2 that resulted in the decrease in the invasion and migration, but not proliferation, of U2OS cells. The expression levels of Slug and ZEB1, the transcription factors involved in epithelial-mesenchymal transition, significantly reduced after TTYH2 silencing. Based on these results, we suggest that TTYH2 may serve as a novel target for the treatment of osteosarcoma.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Neoplasias Óseas
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Osteosarcoma
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Regulación Neoplásica de la Expresión Génica
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Regulación hacia Arriba
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Movimiento Celular
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Proteínas de la Membrana
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Proteínas de Neoplasias
Límite:
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2019
Tipo del documento:
Article