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Semiautomated Small-Scale Purification Method for High-Throughput Expression Analysis of Recombinant Proteins.
Kraft, Edward; Franke, Yvonne; Heeringa, Katharine; Shriver, Stephanie; Zilberleyb, Inna; Kugel, Christine; Dela Vega, Trisha; Wong, Athena; Brillantes, Bobby; Ciferri, Claudio; Dutina, George; Lee, Grace; Lehoux, Isabelle; Li, Zhong Rong; Lior-Hoffmann, Lee; Hwang, Jiyoung; Lonergan, Chris; Martin, Lynn; Mortara, Kyle; Nguyen, Lananh; Payandeh, Jian; Perez, Andrew; Sampang, Jun; Singh, Lovejit; Schroeder, Kurt; Tam, Christine; Ti, Shu; Win, Ye Naing; Bowman, Krista.
Afiliación
  • Kraft E; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Franke Y; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Heeringa K; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Shriver S; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Zilberleyb I; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Kugel C; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Dela Vega T; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Wong A; Department of Early Stage Cell Culture, Genentech, Inc., South San Francisco, CA, USA.
  • Brillantes B; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Ciferri C; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Dutina G; Department of Early Stage Cell Culture, Genentech, Inc., South San Francisco, CA, USA.
  • Lee G; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Lehoux I; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Li ZR; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Lior-Hoffmann L; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Hwang J; 23andMe Inc., South San Francisco, CA, USA.
  • Lonergan C; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Martin L; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Mortara K; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Nguyen L; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Payandeh J; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Perez A; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Sampang J; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Singh L; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Schroeder K; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Tam C; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Ti S; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Win YN; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Bowman K; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA. krafte1@gene.com.
Methods Mol Biol ; 2025: 51-68, 2019.
Article en En | MEDLINE | ID: mdl-31267448
ABSTRACT
The expression analysis of recombinant proteins is a challenging step in any high-throughput protein production pipeline. Often multiple expression systems and a variety of expression construct designs are considered for the production of a protein of interest. There is a strong need to triage constructs rapidly and systematically. This chapter describes a semiautomated method for the simultaneous purification and characterization of proteins expressed from multiple samples of expression cultures from the E. coli, baculovirus expression vector system, and mammalian transient expression systems. This method assists in the selection of the most promising expression construct(s) or the most favorable expression condition(s) to move forward into large-scale protein production.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Proteínas Recombinantes Límite: Animals Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Proteínas Recombinantes Límite: Animals Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos