IFITM3 promotes bone metastasis of prostate cancer cells by mediating activation of the TGF-ß signaling pathway.
Cell Death Dis
; 10(7): 517, 2019 07 04.
Article
en En
| MEDLINE
| ID: mdl-31273201
ABSTRACT
Advanced-stage prostate cancer (PCa) is often diagnosed with bone metastasis, for which there are limited therapies. Transforming growth factor ß (TGF-ß) is known to induce epithelial-mesenchymal transition (EMT), and abundance of TGF-ß in the bone matrix is one of the important growth factors contributing to bone metastasis. TGF-ß is reported as a key mediator of bone metastasis, but the underlying mechanism has not been elucidated. It was found in our study that Interferon-inducible Transmembrane Protein 3 (IFITM3) played a key role in the regulation of malignant tumor cell proliferation, invasion, and bone migration by binding to Smad4, thus activating the TGF-ß-Smads Signaling Pathway. Lentivirus-mediated short hairpin RNA (shRNA) knockdown of IFITM3 inhibited cell proliferation and colony formation, induced apoptosis and inhibited migration by reversing EMT and downregulating the expression of metastasis-related molecules including FGFs and PTHrP. Microarray analysis showed that IFITM3 knockdown could alter the MAPK pathway associated with TGF-ß-Smads signaling. By knocking down and overexpressing IFITM3, we demonstrated that IFITM3 expression level had an effect on MAPK pathway activation, and this change was more pronounced upon exogenous TGF-ß stimulation. These results suggest that IFITM3 played an oncogenic role in PCa progression and bone metastasis via a novel TGF-ß-Smads-MAPK pathway.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Neoplasias de la Próstata
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Neoplasias Óseas
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Transducción de Señal
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Factor de Crecimiento Transformador beta
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Proteínas de Unión al ARN
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Proteínas de la Membrana
Tipo de estudio:
Prognostic_studies
Límite:
Animals
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Humans
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Male
Idioma:
En
Revista:
Cell Death Dis
Año:
2019
Tipo del documento:
Article
País de afiliación:
China