Functional muscle hypertrophy by increased insulin-like growth factor 1 does not require dysferlin.
Muscle Nerve
; 60(4): 464-473, 2019 10.
Article
en En
| MEDLINE
| ID: mdl-31323135
ABSTRACT
INTRODUCTION:
Dysferlin loss-of-function mutations cause muscular dystrophy, accompanied by impaired membrane repair and muscle weakness. Growth promoting strategies including insulin-like growth factor 1 (IGF-1) could provide benefit but may cause strength loss or be ineffective. The objective of this study was to determine whether locally increased IGF-1 promotes functional muscle hypertrophy in dysferlin-null (Dysf-/- ) mice.METHODS:
Muscle-specific transgenic expression and postnatal viral delivery of Igf1 were used in Dysf-/- and control mice. Increased IGF-1 levels were confirmed by enzyme-linked immunosorbent assay. Testing for skeletal muscle mass and function was performed in male and female mice.RESULTS:
Muscle hypertrophy occurred in response to increased IGF-1 in mice with and without dysferlin. Male mice showed a more robust response compared with females. Increased IGF-1 did not cause loss of force per cross-sectional area in Dysf-/- muscles.DISCUSSION:
We conclude that increased local IGF-1 promotes functional hypertrophy when dysferlin is absent and reestablishes IGF-1 as a potential therapeutic for dysferlinopathies.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Factor I del Crecimiento Similar a la Insulina
/
Músculo Esquelético
/
Disferlina
Límite:
Animals
Idioma:
En
Revista:
Muscle Nerve
Año:
2019
Tipo del documento:
Article