Ferritin nanovehicle for targeted delivery of cytochrome C to cancer cells.
Sci Rep
; 9(1): 11749, 2019 08 13.
Article
en En
| MEDLINE
| ID: mdl-31409839
ABSTRACT
In this work, we have exploited the unique properties of a chimeric archaeal-human ferritin to encapsulate, deliver and release cytochrome c and induce apoptosis in a myeloid leukemia cell line. The chimeric protein combines the versatility in 24-meric assembly and cargo incorporation capability of Archaeglobus fulgidus ferritin with specific binding of human H ferritin to CD71, the "heavy duty" carrier responsible for transferrin-iron uptake. Delivery of ferritin-encapsulated cytochrome C to the Acute Promyelocytic Leukemia (APL) NB4 cell line, highly resistant to transfection by conventional methods, was successfully achieved in vitro. The effective liberation of cytochrome C within the cytosolic environment, demonstrated by double fluorescent labelling, induced apoptosis in the cancer cells.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Leucemia Promielocítica Aguda
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Citocromos c
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Nanoestructuras
/
Ferritinas
Límite:
Humans
Idioma:
En
Revista:
Sci Rep
Año:
2019
Tipo del documento:
Article
País de afiliación:
Italia