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Ferritin nanovehicle for targeted delivery of cytochrome C to cancer cells.
Macone, Alberto; Masciarelli, Silvia; Palombarini, Federica; Quaglio, Deborah; Boffi, Alberto; Trabuco, Matilde Cardoso; Baiocco, Paola; Fazi, Francesco; Bonamore, Alessandra.
Afiliación
  • Macone A; Department of Biochemical Sciences "Alessandro Rossi Fanelli", Sapienza University of Rome, Pizzale Aldo Moro 5, 00185, Rome, Italy. alberto.macone@uniroma1.it.
  • Masciarelli S; Department of Anatomical, Histological, Forensic & Orthopaedic Sciences, Section of Histology and Medical Embryology, Sapienza University of Rome, laboratory affiliated to Istituto Pasteur Italia-Fondazione Cenci Bolognetti, Via A. Scarpa, 14-16, 00161, Rome, Italy.
  • Palombarini F; Department of Biochemical Sciences "Alessandro Rossi Fanelli", Sapienza University of Rome, Pizzale Aldo Moro 5, 00185, Rome, Italy.
  • Quaglio D; Department of Chemistry and Technology of Drugs, Sapienza University of Rome, Pizzale Aldo Moro 5, 00185, Rome, Italy.
  • Boffi A; Department of Biochemical Sciences "Alessandro Rossi Fanelli", Sapienza University of Rome, Pizzale Aldo Moro 5, 00185, Rome, Italy.
  • Trabuco MC; Center for Life Nano Science @ Sapienza, Italian Institute of Technology, Viale Regina Elena 291, Rome, 00161, Italy.
  • Baiocco P; Center for Life Nano Science @ Sapienza, Italian Institute of Technology, Viale Regina Elena 291, Rome, 00161, Italy.
  • Fazi F; Department of Anatomical, Histological, Forensic & Orthopaedic Sciences, Section of Histology and Medical Embryology, Sapienza University of Rome, laboratory affiliated to Istituto Pasteur Italia-Fondazione Cenci Bolognetti, Via A. Scarpa, 14-16, 00161, Rome, Italy.
  • Bonamore A; Department of Biochemical Sciences "Alessandro Rossi Fanelli", Sapienza University of Rome, Pizzale Aldo Moro 5, 00185, Rome, Italy. alessandra.bonamore@uniroma1.it.
Sci Rep ; 9(1): 11749, 2019 08 13.
Article en En | MEDLINE | ID: mdl-31409839
ABSTRACT
In this work, we have exploited the unique properties of a chimeric archaeal-human ferritin to encapsulate, deliver and release cytochrome c and induce apoptosis in a myeloid leukemia cell line. The chimeric protein combines the versatility in 24-meric assembly and cargo incorporation capability of Archaeglobus fulgidus ferritin with specific binding of human H ferritin to CD71, the "heavy duty" carrier responsible for transferrin-iron uptake. Delivery of ferritin-encapsulated cytochrome C to the Acute Promyelocytic Leukemia (APL) NB4 cell line, highly resistant to transfection by conventional methods, was successfully achieved in vitro. The effective liberation of cytochrome C within the cytosolic environment, demonstrated by double fluorescent labelling, induced apoptosis in the cancer cells.
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Leucemia Promielocítica Aguda / Citocromos c / Nanoestructuras / Ferritinas Límite: Humans Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article País de afiliación: Italia

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Leucemia Promielocítica Aguda / Citocromos c / Nanoestructuras / Ferritinas Límite: Humans Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article País de afiliación: Italia