Confluentibacter sediminis sp. nov., isolated from the junction between the ocean and a freshwater lake and emended description of the genus Confluentibacter.

ABSTRACT

A novel marine Gram-stain-negative, non-motile, aerobic and rod-shaped bacterium, designated strain DSL-48T, was isolated from tidal flat sediment sampled from the East China Sea and characterized phylogenetically and phenotypically. Bacterial optimal growth occurred at 35 °C (range, 4-37 °C), pH 6 (pH 5-10) and with 4 % (w/v) NaCl (0-7 %). The nearest phylogenetic neighbour was Confluentibacter citreus KCTC 52638T with 16S rRNA gene similarity of 97.1 %. The predominant respiratory quinone was menaquinone-6 (MK-6). The major polar lipids were phosphatidylethanolamine, three unidentified aminolipids and four unidentified lipids. The major fatty acids of strain DSL-48T were iso-C15  0, iso-C17  0 3-OH, anteiso-C15  0, iso-C15  0 3-OH and iso-C16  0 3-OH. The G+C content of the genomic DNA was 33.3 mol%. The combined genotypic and phenotypic data indicated that strain DSL-48T represents a novel species of the genus Confluentibacter, for which the name Confluentibacter sediminis sp. nov. is proposed, with the type strain DSL-48T (=KCTC 62648T=MCCC 1K03537T).