Biosynthesis of GDP-d-glycero-α-d-manno-heptose for the Capsular Polysaccharide of Campylobacter jejuni.
Biochemistry
; 58(37): 3893-3902, 2019 09 17.
Article
en En
| MEDLINE
| ID: mdl-31449400
ABSTRACT
The capsular polysaccharide (CPS) structure of Campylobacter jejuni contributes to its robust fitness. Many strains contain heptose moieties in their CPS units. The precursor heptose is GDP-d-glycero-α-d-manno-heptose; modifications to the stereochemistry at C3-C6 as well as additions of methyl and phosphoramidate groups lend to the hypervariability of the C. jejuni CPS structures. Synthesis of GDP-d-glycero-α-d-manno-heptose has been described previously, but using enzymes from Aneurinibacillus thermoaerophilus DSM 10155. Here we describe the complete synthesis of GDP-d-glycero-α-d-manno-heptose using enzymes from C. jejuni NTCC 11168 Cj1152 and Cj1423-Cj1425. Our results yield kinetic parameters for these enzymes and outline a successful strategy for milligram-gram scale synthesis of GDP-d-glycero-α-d-manno-heptose. This achievement is critical for the characterization of other carbohydrate tailoring enzymes, which are expected to utilize GDP-d-glycero-α-d-manno-heptose for the biosynthesis of more complex carbohydrates in the CPS of C. jejuni.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Polisacáridos
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Proteínas Bacterianas
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Campylobacter jejuni
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Cápsulas Bacterianas
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Guanosina Difosfato
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Heptosas
Idioma:
En
Revista:
Biochemistry
Año:
2019
Tipo del documento:
Article
País de afiliación:
Estados Unidos