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CRISPR-Cas9-assisted native end-joining editing offers a simple strategy for efficient genetic engineering in Escherichia coli.
Huang, Chaoyong; Ding, Tingting; Wang, Jingge; Wang, Xueqin; Guo, Liwei; Wang, Jialei; Zhu, Lin; Bi, Changhao; Zhang, Xueli; Ma, Xiaoyan; Huo, Yi-Xin.
Afiliación
  • Huang C; Key Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Beijing, 100081, People's Republic of China.
  • Ding T; Key Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Beijing, 100081, People's Republic of China.
  • Wang J; UCLA (Suzhou) Institute for Technology Advancement, 10 Yueliangwan Road, Suzhou Industrial Park, Suzhou, 215123, People's Republic of China.
  • Wang X; Key Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Beijing, 100081, People's Republic of China.
  • Guo L; Key Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Beijing, 100081, People's Republic of China.
  • Wang J; Key Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Beijing, 100081, People's Republic of China.
  • Zhu L; Key Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Beijing, 100081, People's Republic of China.
  • Bi C; Key Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Beijing, 100081, People's Republic of China.
  • Zhang X; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China.
  • Ma X; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China.
  • Huo YX; Key Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Beijing, 100081, People's Republic of China.
Appl Microbiol Biotechnol ; 103(20): 8497-8509, 2019 Oct.
Article en En | MEDLINE | ID: mdl-31501938
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Ingeniería Genética / Escherichia coli / Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas / Proteína 9 Asociada a CRISPR / Genética Microbiana Idioma: En Revista: Appl Microbiol Biotechnol Año: 2019 Tipo del documento: Article
Texto completo
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Ingeniería Genética / Escherichia coli / Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas / Proteína 9 Asociada a CRISPR / Genética Microbiana Idioma: En Revista: Appl Microbiol Biotechnol Año: 2019 Tipo del documento: Article