Immune recovery following bronchiolitis is linked to a drop in cytokine and LTC4 levels.
Pediatr Res
; 87(3): 581-587, 2020 02.
Article
en En
| MEDLINE
| ID: mdl-31600771
ABSTRACT
BACKGROUND:
Bronchiolitis is the main cause of hospitalization of children younger than 1 year; however, the immune mechanism of bronchiolitis is not completely understood. The aim of this study was to analyze the recovery of immune response after a bronchiolitis episode.METHODS:
Forty-nine infants hospitalized with bronchiolitis diagnosis were enrolled. Nasopharyngeal aspirates (NPAs) were processed. Twenty-seven pro-inflammatory biomarkers linked to innate immunity, inflammation, and epithelial damage, as well as nitrites and lipid mediators, were evaluated in the NPA supernatant by ELISA (enzyme-linked immunosorbent assay) and Luminex. Also, 11 genes were analyzed in NPA cells by quantitative PCR.RESULTS:
A widespread statistically significant decline of multiple pro-inflammatory parameters and cytokines were detected in the recovery period after respiratory infection interferon-α2 (IFNα2), IFNγ, interleukin-10 (IL-10), IL-1ß, IL-8, IFN-γ-inducible protein-10, vascular endothelial growth factor, monocyte chemoattractant protein-1, macrophage inflammatory protein-1α (MIP-1α), and MIP-1ß. Supporting these results, a decreased nuclear factor-κB gene expression was observed (P = 0.0116). A significant diminution of cysteinyl leukotriene C4 (LTC4) soluble levels (P = 0.0319) and cyclooxygenase-2 (COX-2) gene expression were observed in the recovery sample. In children classified by post-bronchiolitis wheezing, LTC4 remains elevated in the NPA supernatant.CONCLUSIONS:
After bronchiolitis, cytokines and biomarkers linked to innate immune response in NPA decrease significantly in the recovery period accompanied by a drop in LTC4 levels; however, this reduction was lower in infants with post-bronchiolitis wheezing.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Bronquiolitis
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Nasofaringe
/
Citocinas
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Leucotrieno C4
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Inmunidad Adaptativa
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Inmunidad Innata
Tipo de estudio:
Diagnostic_studies
/
Observational_studies
Límite:
Female
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Humans
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Infant
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Male
Idioma:
En
Revista:
Pediatr Res
Año:
2020
Tipo del documento:
Article
País de afiliación:
España