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Cryo-Gel embedding compound for renal biopsy biobanking.
Snijders, Malou L H; Zajec, Marina; Walter, Laurens A J; de Louw, Remco M A A; Oomen, Monique H A; Arshad, Shazia; van den Bosch, Thierry P P; Dekker, Lennard J M; Doukas, Michail; Luider, Theo M; Riegman, Peter H J; van Kemenade, Folkert J; Clahsen-van Groningen, Marian C.
Afiliación
  • Snijders MLH; Department of Pathology, Erasmus MC, Rotterdam, The Netherlands. m.snijders@erasmusmc.nl.
  • Zajec M; Department of Neurology, Erasmus MC, Rotterdam, The Netherlands.
  • Walter LAJ; Department of Clinical Chemistry, Erasmus MC, Rotterdam, The Netherlands.
  • de Louw RMAA; Department of Pathology, Erasmus MC, Rotterdam, The Netherlands.
  • Oomen MHA; Department of Pathology, Erasmus MC, Rotterdam, The Netherlands.
  • Arshad S; Department of Pathology, Erasmus MC, Rotterdam, The Netherlands.
  • van den Bosch TPP; Department of Pathology, Erasmus MC, Rotterdam, The Netherlands.
  • Dekker LJM; Department of Pathology, Erasmus MC, Rotterdam, The Netherlands.
  • Doukas M; Department of Neurology, Erasmus MC, Rotterdam, The Netherlands.
  • Luider TM; Department of Pathology, Erasmus MC, Rotterdam, The Netherlands.
  • Riegman PHJ; Department of Neurology, Erasmus MC, Rotterdam, The Netherlands.
  • van Kemenade FJ; Department of Pathology, Erasmus MC, Rotterdam, The Netherlands.
  • Clahsen-van Groningen MC; Department of Pathology, Erasmus MC, Rotterdam, The Netherlands.
Sci Rep ; 9(1): 15250, 2019 10 24.
Article en En | MEDLINE | ID: mdl-31649317
Optimal preservation and biobanking of renal tissue is vital for good diagnostics and subsequent research. Optimal cutting temperature (OCT) compound is a commonly used embedding medium for freezing tissue samples. However, due to interfering polymers in OCT, analysis as mass spectrometry (MS) is difficult. We investigated if the replacement of OCT with Cryo-Gel as embedding compound for renal biopsies would enable proteomics and not disturb other common techniques used in tissue diagnostics and research. For the present study, fresh renal samples were snap-frozen using Cryo-Gel, OCT and without embedding compound and evaluated using different techniques. In addition, tissue samples from normal spleen, skin, liver and colon were analyzed. Cryo-Gel embedded tissues showed good morphological preservation and no interference in immunohistochemical or immunofluorescent investigations. The quality of extracted RNA and DNA was good. The number of proteins identified using MS was similar between Cryo-Gel embedded samples, samples without embedding compound and OCT embedded samples. However, polymers in the OCT disturbed the signal in the MS, while this was not observed in the Cryo-Gel embedded samples. We conclude that embedding of renal biopsies in Cryo-Gel is an excellent and preferable alternative for OCT compound for both diagnostic and research purposes, especially in those cases where proteomic analysis might be necessary.
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Adhesión del Tejido / Bancos de Muestras Biológicas / Riñón Límite: Humans Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article País de afiliación: Países Bajos

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Adhesión del Tejido / Bancos de Muestras Biológicas / Riñón Límite: Humans Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article País de afiliación: Países Bajos