Identification of an Apis cerana cerana MAP kinase phosphatase 3 gene (AccMKP3) in response to environmental stress.
Cell Stress Chaperones
; 24(6): 1137-1149, 2019 11.
Article
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| MEDLINE
| ID: mdl-31664697
ABSTRACT
MAP kinase phosphatase 3 (MKP3), a member of the dual-specificity protein phosphatase (DUSP) superfamily, has been widely studied for its role in development, cancer, and environmental stress in many organisms. However, the functions of MKP3 in various insects have not been well studied, including honeybees. In this study, we isolated an MKP3 gene from Apis cerana cerana and explored the role of this gene in the resistance to oxidation. We found that AccMKP3 is highly conserved in different species and shares the closest evolutionary relationship with AmMKP3. We determined the expression patterns of AccMKP3 under various stresses. qRT-PCR results showed that AccMKP3 was highly expressed during the pupal stages and in adult muscles. We further found that AccMKP3 was induced in all the stress treatments. Moreover, we discovered that the enzymatic activities of peroxidase, superoxide dismutase, and catalase increased and that the expression levels of several antioxidant genes were affected after AccMKP3 was knocked down. Collectively, these results suggest that AccMKP3 may be associated with antioxidant processes involved in response to various environmental stresses.
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Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Estrés Fisiológico
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Abejas
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Genes de Insecto
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Estrés Oxidativo
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Proteínas de Insectos
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Fosfatasa 6 de Especificidad Dual
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Cell Stress Chaperones
Año:
2019
Tipo del documento:
Article