Mycobacterium abscessus virulence traits unraveled by transcriptomic profiling in amoeba and macrophages.
PLoS Pathog
; 15(11): e1008069, 2019 11.
Article
en En
| MEDLINE
| ID: mdl-31703112
ABSTRACT
Free-living amoebae are thought to represent an environmental niche in which amoeba-resistant bacteria may evolve towards pathogenicity. To get more insights into factors playing a role for adaptation to intracellular life, we characterized the transcriptomic activities of the emerging pathogen Mycobacterium abscessus in amoeba and murine macrophages (MÏ) and compared them with the intra-amoebal transcriptome of the closely related, but less pathogenic Mycobacterium chelonae. Data on up-regulated genes in amoeba point to proteins that allow M. abscessus to resist environmental stress and induce defense mechanisms, as well as showing a switch from carbohydrate carbon sources to fatty acid metabolism. For eleven of the most upregulated genes in amoeba and/or MÏ, we generated individual gene knock-out M. abscessus mutant strains, from which ten were found to be attenuated in amoeba and/or MÏ in subsequence virulence analyses. Moreover, transfer of two of these genes into the genome of M. chelonae increased the intra-MÏ survival of the recombinant strain. One knock-out mutant that had the gene encoding Eis N-acetyl transferase protein (MAB_4532c) deleted, was particularly strongly attenuated in MÏ. Taken together, M. abscessus intra-amoeba and intra-MÏ transcriptomes revealed the capacity of M. abscessus to adapt to an intracellular lifestyle, with amoeba largely contributing to the enhancement of M. abscessus intra-MÏ survival.
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1
Bases de datos:
MEDLINE
Asunto principal:
Virulencia
/
Factores de Virulencia
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Transcriptoma
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Mycobacterium abscessus
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Amoeba
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Macrófagos
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Infecciones por Mycobacterium no Tuberculosas
Límite:
Animals
Idioma:
En
Revista:
PLoS Pathog
Año:
2019
Tipo del documento:
Article
País de afiliación:
Francia