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Characterization of an Adapted Murine Model of Intrauterine Inflammation-Induced Preterm Birth.
Zierden, Hannah C; Ortiz Ortiz, Jairo I; Dimitrion, Peter; Laney, Victoria; Bensouda, Sabrine; Anders, Nicole M; Scardina, Morgan; Hoang, Thuy; Ronnett, Brigitte M; Hanes, Justin; Burd, Irina; Mahendroo, Mala; Ensign, Laura M.
Afiliación
  • Zierden HC; The Center for Nanomedicine, The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland.
  • Ortiz Ortiz JI; The Center for Nanomedicine, The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland; Department of Ophthalmology, The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland.
  • Dimitrion P; The Center for Nanomedicine, The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland.
  • Laney V; The Center for Nanomedicine, The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland.
  • Bensouda S; The Center for Nanomedicine, The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland.
  • Anders NM; The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland.
  • Scardina M; The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland.
  • Hoang T; The Center for Nanomedicine, The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland; Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland.
  • Ronnett BM; Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, Maryland.
  • Hanes J; The Center for Nanomedicine, The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland; Department of Ophthalmology, The Wilmer Eye Institute, Johns Hopkins University
  • Burd I; Division of Maternal Fetal Medicine, Department of Gynecology and Obstetrics, Integrated Research Center for Fetal Medicine, Johns Hopkins University, Baltimore, Maryland.
  • Mahendroo M; Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas, Texas.
  • Ensign LM; The Center for Nanomedicine, The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland; Department of Ophthalmology, The Wilmer Eye Institute, Johns Hopkins University
Am J Pathol ; 190(2): 295-305, 2020 02.
Article en En | MEDLINE | ID: mdl-31837289
ABSTRACT
Preterm birth (PTB) affects nearly 15 million infants each year. Of these PTBs, >25% are a result of inflammation or infection. Animal models have improved our understanding of the mechanisms leading to PTB. Prior work has described induction of intrauterine inflammation in mice with a single injection of lipopolysaccharide (LPS). Herein, we have improved the reproducibility and potency of LPS in the model using two injections distal to the cervix. An in vivo imaging system revealed more uniform distribution of Evans Blue Dye using a double distal injection (DDI) approach compared with a single proximal injection (SPI). Endotoxin concentrations in vaginal lavage fluid from SPI dams were significantly higher than from DDI dams. At equivalent LPS doses, DDI consistently induced more PTB than SPI, and DDI showed a linear dose-response, whereas SPI did not. Gene expression in myometrial tissue revealed increased levels of inflammatory markers in dams that received LPS DDI compared with LPS SPI. The SPI group showed more significant overexpression in cervical remodeling genes, likely due to the leakage of LPS from the uterine horns through the cervix. The more reliable PTB induction and uniform uterine exposure provided by this new model will be useful for further studying fetal outcomes and potential therapeutics for the prevention of inflammation-induced PTB.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Efectos Tardíos de la Exposición Prenatal / Lipopolisacáridos / Nacimiento Prematuro / Modelos Animales de Enfermedad / Inflamación / Miometrio Límite: Animals / Pregnancy Idioma: En Revista: Am J Pathol Año: 2020 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Efectos Tardíos de la Exposición Prenatal / Lipopolisacáridos / Nacimiento Prematuro / Modelos Animales de Enfermedad / Inflamación / Miometrio Límite: Animals / Pregnancy Idioma: En Revista: Am J Pathol Año: 2020 Tipo del documento: Article