Increased hydrostatic pressure induces nuclear translocation of DAF-16/FOXO in C. elegans.
Biochem Biophys Res Commun
; 523(4): 853-858, 2020 03 19.
Article
en En
| MEDLINE
| ID: mdl-31954516
ABSTRACT
Mechanical stimulation is well known to be important for maintaining tissue and organ homeostasis. Here, we found that hydrostatic pressure induced nuclear translocation of a forkhead box O (FOXO) transcription factor DAF-16, in C. elegans within minutes, whereas the removal of this pressure resulted in immediate export of DAF-16 to the cytoplasm. We also monitored DAF-16-dependent transcriptional changes by exposure to 1 MPa pressure for 5 min, and found significant changes in collagen and other genes in a DAF-16 dependent manner. Lifespan was markedly prolonged with exposure to cyclic pressure treatment (1 MPa once a day for 5 min from L1 larvae until death). Furthermore, age-dependent decline in locomotor activity was suppressed by the treatment. In contrast, the nuclear translocation of the yes-associated protein YAP-1 was not induced under the same pressure conditions. Thus, moderate hydrostatic pressure improves ageing progression through activation of DAF-16/FOXO in C. elegans.
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Texto completo:
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Bases de datos:
MEDLINE
Asunto principal:
Núcleo Celular
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Caenorhabditis elegans
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Proteínas de Caenorhabditis elegans
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Factores de Transcripción Forkhead
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Presión Hidrostática
Límite:
Animals
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En
Revista:
Biochem Biophys Res Commun
Año:
2020
Tipo del documento:
Article