Site-directed labeling of ß-arrestin with monobromobimane for measuring their interaction with G protein-coupled receptors.

Srivastava, Ashish; Baidya, Mithu; Dwivedi-Agnihotri, Hemlata; Shukla, Arun K

Srivastava, Ashish; Baidya, Mithu; Dwivedi-Agnihotri, Hemlata; Shukla, Arun K.

Afiliación

Srivastava A; Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, India.
Baidya M; Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, India.
Dwivedi-Agnihotri H; Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, India.
Shukla AK; Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, India. Electronic address: arshukla@iitk.ac.in.

Methods Enzymol ; 633: 271-280, 2020.

Article en En | MEDLINE | ID: mdl-32046850

Asunto(s)

Biopelículas; Reactores Biológicos; Compuestos Bicíclicos con Puentes; Receptores Acoplados a Proteínas G; beta-Arrestinas; Proteínas de Unión al GTP; Receptores Acoplados a Proteínas G/metabolismo; beta-Arrestinas/metabolismo

Palabras clave

Biased agonism; Bimane; Drug discovery; Fluorescence spectroscopy; GPCRs; Proteinprotein interaction; ß-Arrestins

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Compuestos Bicíclicos con Puentes / Biopelículas / Reactores Biológicos / Receptores Acoplados a Proteínas G / Beta-Arrestinas Idioma: En Revista: Methods Enzymol Año: 2020 Tipo del documento: Article País de afiliación: India

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