Regeneration of sciatic nerves by transplanted microvesicles of human neural stem cells derived from embryonic stem cells.
Cell Tissue Bank
; 21(2): 233-248, 2020 Jun.
Article
en En
| MEDLINE
| ID: mdl-32052220
ABSTRACT
Injured nerves cannot regenerate on their own, and a lack of engraftable human nerves has been a major obstacle in cell-based therapies for regenerating damaged nerves. A monolayer culture approach to obtain adherent neural stem cells from human embryonic stem cells (hESC-NSCs) was established, and the greatest number of stemness characteristics were achieved by the eighth generation of hESC-NSCs (P8 hESC-NSCs). To overcome deficits in cell therapy, we used microvesicles secreted from P8 hESC-NSCs (hESC-NSC-MVs) instead of entire hESC-NSCs. To investigate the therapeutic efficacy of hESC-NSC-MVs in vitro, hESC-NSC-MVs were cocultured with dorsal root ganglia to determine the length of axons. In vivo, we transected the sciatic nerve in SD rats and created a 5-mm gap. A sciatic nerve defect was bridged using a silicone tube filled with hESC-NSC-MVs (45 µg) in the MVs group, P8 hESC-NSCs (1 × 106 single cells) in the cell group and PBS in the control group. The hESC-NSC-MVs group showed better morphological recovery and a significantly greater number of regenerated axons than the hESC-NSCs group 12 weeks after nerve injury. These results indicated that the hESC-NSC-MVs group had the greatest ability to repair and reconstruct nerve structure and function. As a result, hESC-NSC-MVs may have potential for applications in the field of nerve regenerative repair.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Nervio Ciático
/
Micropartículas Derivadas de Células
/
Células-Madre Neurales
/
Células Madre Embrionarias Humanas
/
Regeneración Nerviosa
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Cell Tissue Bank
Asunto de la revista:
HISTOLOGIA
/
TRANSPLANTE
Año:
2020
Tipo del documento:
Article
País de afiliación:
China