A reporter cell line for real-time imaging of autophagy and apoptosis.
Toxicol Lett
; 326: 23-30, 2020 Jun 15.
Article
en En
| MEDLINE
| ID: mdl-32109534
ABSTRACT
Simultaneous detection of autophagy and apoptosis is important in drug discovery and signaling studies. Here we report, a real-time reporter cell line for the simultaneous detection of apoptosis and autophagy at single-cell level employing stable integration of two fluorescent protein reporters of apoptosis and autophagy. Cells stably expressing EGFP-LC3 fusion was developed initially as a marker for autophagy and subsequently stably expressed with inter-mitochondrial membrane protein SMAC with RFP fusion to detect mitochondrial permeabilization event of apoptosis. The cell lines faithfully reported the LC3 punctae formation and release of intermembrane proteins in response to diverse apoptotic and autophagic stimuli.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Autofagia
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Células HeLa
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Apoptosis
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Genes Reporteros
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Línea Celular Tumoral
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Proteínas Fluorescentes Verdes
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Evaluación Preclínica de Medicamentos
Límite:
Humans
Idioma:
En
Revista:
Toxicol Lett
Año:
2020
Tipo del documento:
Article
País de afiliación:
India