Rapid mesoscale volumetric imaging of neural activity with synaptic resolution.
Nat Methods
; 17(3): 291-294, 2020 03.
Article
en En
| MEDLINE
| ID: mdl-32123393
ABSTRACT
Imaging neurons and neural circuits over large volumes at high speed and subcellular resolution is a difficult task. Incorporating a Bessel focus module into a two-photon fluorescence mesoscope, we achieved rapid volumetric imaging of neural activity over the mesoscale with synaptic resolution. We applied the technology to calcium imaging of entire dendritic spans of neurons as well as neural ensembles within multiple cortical regions over two hemispheres of the awake mouse brain.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Sinapsis
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Encéfalo
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Dendritas
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Microscopía de Fluorescencia por Excitación Multifotónica
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Neuronas
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos