The impact of PUFA on cell responses: Caution should be exercised when selecting PUFA concentrations in cell culture.
Prostaglandins Leukot Essent Fatty Acids
; 155: 102083, 2020 04.
Article
en En
| MEDLINE
| ID: mdl-32126480
ABSTRACT
Polyunsaturated fatty acids (PUFA) are important components of cellular membranes, serving both structural and signaling functions. Investigation of the functional responses of cells to various PUFA often involves cell culture experiments, which can then inform or guide subsequent in vivo and clinical investigations. In this study, human carcinoma and leukemia cell lines (MCF-7, HepG2, THP-1, Jurkat) were incubated for 3 days in the presence of up to 150 µM of exogenous arachidonic or eicosapentaenoic acids. At concentrations up to 20 µM these PUFA were enriched in cellular phospholipids, but at concentrations of 20 µM or higher cells accumulated large quantities of these PUFA and their elongation products into triglycerides. This coincided with decreased cell proliferation and enhanced apoptosis. Inhibition of DGAT1 but not DGAT2 enhanced the cytotoxic effect of exogenous PUFA suggesting a protective role of PUFA sequestration into TGs. Lower (10 µM) and higher (50 µM) exogenous PUFA concentrations also had different impacts on the expression of PUFA metabolizing enzymes. Overall, these results indicate that caution must be exercised when planning in vitro experiments since elevated concentrations of PUFA can lead to dysfunctional cellular responses that are not predictive of in vivo responses to dietary PUFA.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Ácido Eicosapentaenoico
/
Ácido Araquidónico
/
Técnicas de Cultivo de Célula
/
Sustancias Protectoras
Límite:
Humans
Idioma:
En
Revista:
Prostaglandins Leukot Essent Fatty Acids
Asunto de la revista:
ENDOCRINOLOGIA
Año:
2020
Tipo del documento:
Article
País de afiliación:
Canadá