Broadening the spectrum of fluorescent protein tools for use in the encapsulated human fungal pathogen Cryptococcus neoformans.
Fungal Genet Biol
; 138: 103365, 2020 05.
Article
en En
| MEDLINE
| ID: mdl-32145317
ABSTRACT
Green fluorescent protein (GFP) and its counterparts are modern molecular biology research tools indispensable in many experimental systems. Within fungi, researchers studying Saccharomyces cerevisiae and other model ascomycetes have access to a wide variety of fluorescent proteins. Unfortunately, many of these tools have not crossed the phylum divide into the Basidiomycota, where only GFP S65T, Venus, Ds-Red, and mCherry are currently available. To address this, we searched the literature for potential candidates to be expressed in the human fungal pathogen Cryptococcus neoformans and identified a suite of eight more modern fluorescent proteins that span the visible spectrum. A single copy of each fluorophore was heterologously expressed in Safe Haven 1 and their fluorescence intensities compared in this encapsulated yeast. mTurquoise2, mTFP1, Clover, mNeonGreen, mRuby3, and Citrine were highly visible under the microscope, whereas Superfolder GFP and mMaroon1 were not. Expressed fluorophores did not impact growth or virulence as demonstrated by an in vitro spotting assay and murine inhalation model, respectively.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Cryptococcus neoformans
/
Colorantes Fluorescentes
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Fungal Genet Biol
Asunto de la revista:
GENETICA
/
MICROBIOLOGIA
Año:
2020
Tipo del documento:
Article
País de afiliación:
Australia