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Quaternized styryl-azinium fluorophores as cellular RNA-binders.
Botti, Valentina; Urbanelli, Lorena; Sagini, Krizia; Tarpani, Luigi; Cesaretti, Alessio; Fortuna, Cosimo G; Elisei, Fausto.
Afiliación
  • Botti V; Department of Chemistry, Biology and Biotechnology and Center of Excellence on Innovative Nanostructured Materials (CEMIN), University of Perugia, via Elce di Sotto 8, 06123, Perugia, Italy. valentinabotti.90@gmail.com.
  • Urbanelli L; Department of Chemistry, Biology and Biotechnology, University of Perugia, via del Giochetto, 06126, Perugia, Italy.
  • Sagini K; Department of Chemistry, Biology and Biotechnology, University of Perugia, via del Giochetto, 06126, Perugia, Italy.
  • Tarpani L; Department of Chemistry, Biology and Biotechnology and Center of Excellence on Innovative Nanostructured Materials (CEMIN), University of Perugia, via Elce di Sotto 8, 06123, Perugia, Italy.
  • Cesaretti A; Department of Chemistry, Biology and Biotechnology and Center of Excellence on Innovative Nanostructured Materials (CEMIN), University of Perugia, via Elce di Sotto 8, 06123, Perugia, Italy.
  • Fortuna CG; Department of Chemical Sciences, University of Catania, viale Andrea Doria 6, 95125, Catania, Italy.
  • Elisei F; Department of Chemistry, Biology and Biotechnology and Center of Excellence on Innovative Nanostructured Materials (CEMIN), University of Perugia, via Elce di Sotto 8, 06123, Perugia, Italy.
Photochem Photobiol Sci ; 19(3): 362-370, 2020 Mar 01.
Article en En | MEDLINE | ID: mdl-32147676
ABSTRACT
The capability of three quaternized styryl-azinium iodides to bind cellular RNA has been tested by means of Fluorescence Confocal Microscopy imaging of stained MCF-7 cells treated with RNase. Their association constants have been estimated through spectrophotometric and fluorimetric titrations with tRNA and compared to their affinity toward DNA. Transient absorption spectroscopy with femtosecond resolution confirmed the binding of the investigated compounds with tRNA and shed new light on the excited state dynamics of their complexes, by revealing a significant lengthening of the lifetime of S1 upon complexation, which parallels the fluorescence quantum yield enhancement.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Pirazinas / Estirenos / ARN / Colorantes Fluorescentes Límite: Humans Idioma: En Revista: Photochem Photobiol Sci Asunto de la revista: BIOLOGIA / QUIMICA Año: 2020 Tipo del documento: Article País de afiliación: Italia
Texto completo
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XML
PubMed Links
Buscar en Google

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Pirazinas / Estirenos / ARN / Colorantes Fluorescentes Límite: Humans Idioma: En Revista: Photochem Photobiol Sci Asunto de la revista: BIOLOGIA / QUIMICA Año: 2020 Tipo del documento: Article País de afiliación: Italia