A new fluorescent probe for sensing of biothiols and screening of acetylcholinesterase inhibitors.
Org Biomol Chem
; 18(13): 2468-2474, 2020 04 01.
Article
en En
| MEDLINE
| ID: mdl-32167516
ABSTRACT
A new N2O-type BODIPY probe (LF-Bop) has been proposed for the selective and sensitive detection of biologically relevant small molecular thiols. This detection is based on the Michael addition reaction between the thiol and nitrostyrene groups in the probe, which decreases the quenching effect from the nitro group, thus resulting in the recovery of the deep-red fluorescence from the BODIPY structure. The results show that LF-Bop is able to detect all tested free thiols through a fluorescence turn-on assay. The lowest limit of detection (LOD) for glutathione was found to be down to nanomolar levels (220 nM). Based on this probe, we have developed a new fluorescence assay for the screening of acetylcholinesterase inhibitors. In total, 11 natural and synthetic alkaloids have been evaluated. Both experimental measurements and theoretical molecular docking results reveal that both natural berberine and its synthetic derivative dihydroberberine are potential inhibitors of acetylcholinesterase.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Estirenos
/
Compuestos de Boro
/
Inhibidores de la Colinesterasa
/
Colorantes Fluorescentes
/
Glutatión
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Límite:
Animals
Idioma:
En
Revista:
Org Biomol Chem
Asunto de la revista:
BIOQUIMICA
/
QUIMICA
Año:
2020
Tipo del documento:
Article