Production of a specific monoclonal antibody for 1-naphthol based on novel hapten strategy and development of an easy-to-use ELISA in urine samples.
Ecotoxicol Environ Saf
; 196: 110533, 2020 Jun 15.
Article
en En
| MEDLINE
| ID: mdl-32247241
ABSTRACT
1-naphthol (1-NAP) is the main metabolite of pesticide carbaryl and naphthalene, and is also a genotoxic and carcinogenic intermediate in the synthesis of organic compound, dyes, pigment and pharmaceutical industry. In this work, two novel haptens were designed and synthesized for developing a competitive indirect enzyme-linked immunosorbent assay (ciELISA) method for 1-NAP in urine samples. The assay showed a limit of detection of 2.21 ng/mL and working range from 4.02 ng/mL to 31.25 ng/mL for 1-NAP in optimized working buffer. The matrix effect of samples was eliminated via 15-fold dilution of optimized working buffer. Good average recoveries (102.4%-123.4%) with a coefficient of variation from 11.7% to 14.7% was obtained for spiked urine samples. Subsequent instrument verification test showed good correlation between the results of ciELISA and high-performance liquid chromatography. The developed ciELISA is a high-throughput tool to monitor 1-NAP in urine, which can provide technical support for the establishment of biological exposure level for the exposure to carbaryl, naphthalene and other related pollutants.
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Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Ensayo de Inmunoadsorción Enzimática
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Residuos de Plaguicidas
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Haptenos
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Anticuerpos Monoclonales
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Naftoles
Idioma:
En
Revista:
Ecotoxicol Environ Saf
Año:
2020
Tipo del documento:
Article