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Experimental Models of Partial Intestinal Obstruction in Young Mice: Establishment, and Evaluation.
Georgopoulos, Ioannis; Mavrigiannaki, Eleftheria; Stasinopoulou, Sotiria; Renieris, Georgios; Nikolakis, Georgios; Chaniotakis, Ioannis; Tiniakos, Dina; Papaconstantinou, Ioannis.
Afiliación
  • Georgopoulos I; Department of Pediatric Surgery, 'Agia Sofia' Children's Hospital, Athens, Greece. Electronic address: ipgeorgopoulos@gmail.com.
  • Mavrigiannaki E; Department of Pediatric Surgery, 'Agia Sofia' Children's Hospital, Athens, Greece.
  • Stasinopoulou S; Department of Pathology, 417 Veterans Administration Hospital (NIMTS), Athens, Greece.
  • Renieris G; 4th Department of Internal Medicine, National and Kapodistrian University of Athens, Medical School, Athens, Greece.
  • Nikolakis G; Departments of Dermatology, Venereology, Allergology and Immunology, Dessau Medical Center, Medical School Brandenburg Theodor Fontane, Dessau, Germany.
  • Chaniotakis I; Veterinary Support Department Healthcare Directorate of Hellenic Air Force General Staff (HAFGS), Athens, Greece.
  • Tiniakos D; Department of Pathology, Aretaieion Hospital, Medical School, National & Kapodistrian University of Athens, Athens, Greece; Faculty of Medical Sciences, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, United Kingdom.
  • Papaconstantinou I; Second Department of Surgery, National and Kapodistrian University of Athens, Medical School, Aretaieion University Hospital, Athens, Greece.
J Surg Res ; 252: 206-215, 2020 08.
Article en En | MEDLINE | ID: mdl-32283334
BACKGROUND: Partial small bowel obstruction (SBO) is a common, potentially hazardous, surgical entity caused by numerous factors in humans. A number of techniques have been reported as efficient to simulate partial SBO in murine models. However, there is little data concerning their long-term survival. Our study presents a novel technique and evaluates its long-term efficiency compared with other commonly used techniques. MATERIALS AND METHODS: Sixty C57BL/6 mice aged 6 to 8 wk were randomly divided into five intervention groups: ligation, intestinal ring, partial ligation, microclips, and the novel triple suture technique. The ring groups were subdivided into narrow, medium, and wide ring and partial ligation groups were subdivided at 1/3, 1/2, and 2/3 of the lumen. Survival cutoff time was set at 4 wk. Animals were then euthanized and small bowel muscle layer thickness was histopathologically evaluated. RESULTS: None of the animals of the ligation and the ring groups reached the cutoff survival time. The mortality rate of the partial ligation and the microclips groups at the 4-week period were 33.3% and 0%, respectively. However, elimination of the performed intervention was revealed at the time of euthanasia and no alterations of the muscle layer were revealed at histopathology. The "triple suture" group had a survival rate of 90% until euthanasia and the sutures were apparent in all cases. Macroscopic evaluation showed small to mild proximal lumen dilatation in 6 of 10 animals. Histopathological evaluation of the specimens confirmed the partial obstruction. CONCLUSIONS: The "triple suture" technique is a new, robust, reliable, and inexpensive technique for experimental long-standing partial SBO, with very low mortality.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Modelos Animales de Enfermedad / Obstrucción Intestinal / Intestino Delgado Tipo de estudio: Clinical_trials / Evaluation_studies / Prognostic_studies Límite: Animals / Female / Humans / Male Idioma: En Revista: J Surg Res Año: 2020 Tipo del documento: Article

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Modelos Animales de Enfermedad / Obstrucción Intestinal / Intestino Delgado Tipo de estudio: Clinical_trials / Evaluation_studies / Prognostic_studies Límite: Animals / Female / Humans / Male Idioma: En Revista: J Surg Res Año: 2020 Tipo del documento: Article