Simultaneous measurement of biochemical phenotypes and gene expression in single cells.

Richer, Amanda L; Riemondy, Kent A; Hardie, Lakotah; Hesselberth, Jay R

Richer, Amanda L; Riemondy, Kent A; Hardie, Lakotah; Hesselberth, Jay R.

Afiliación

Richer AL; Department of Biochemistry and Molecular Genetics, Aurora, CO 80045, USA.
Riemondy KA; Molecular Biology Program.
Hardie L; RNA Bioscience Initiative, University of Colorado School of Medicine, Aurora, CO 80045, USA.
Hesselberth JR; Department of Biochemistry and Molecular Genetics, Aurora, CO 80045, USA.

Nucleic Acids Res ; 48(10): e59, 2020 06 04.

Article en En | MEDLINE | ID: mdl-32286626

RESUMEN

Methods to measure heterogeneity among cells are rapidly transforming our understanding of biology but are currently limited to molecular abundance measurements. We developed an approach to simultaneously measure biochemical activities and mRNA abundance in single cells to understand the heterogeneity of DNA repair activities across thousands of human lymphocytes, identifying known and novel cell-type-specific DNA repair phenotypes.

Asunto(s)

Reparación del ADN; Expresión Génica; Análisis de la Célula Individual/métodos; Línea Celular; Genómica; Secuenciación de Nucleótidos de Alto Rendimiento; Humanos; Linfocitos/metabolismo; Fenotipo; ARN Mensajero/química; ARN Mensajero/metabolismo; RNA-Seq; Análisis de Secuencia de ADN

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Expresión Génica / Reparación del ADN / Análisis de la Célula Individual Límite: Humans Idioma: En Revista: Nucleic Acids Res Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos

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