Kanamycin Uptake into Escherichia coli Is Facilitated by OmpF and OmpC Porin Channels Located in the Outer Membrane.
ACS Infect Dis
; 6(7): 1855-1865, 2020 07 10.
Article
en En
| MEDLINE
| ID: mdl-32369342
ABSTRACT
Despite decades of therapeutic application of aminoglycosides, it is still a matter of debate if porins contribute to the translocation of the antibiotics across the bacterial outer membrane. Here, we quantified the uptake of kanamycin across the major porin channels OmpF and OmpC present in the outer membrane of Escherichia coli. Our analysis revealed that, despite its relatively large size, about 10-20 kanamycin molecules per second permeate through OmpF and OmpC under a 10 µM concentration gradient, whereas OmpN does not allow the passage. Molecular simulations elucidate the uptake mechanism of kanamycin through these porins. Whole-cell studies with a defined set of E. coli porin mutants provide evidence that translocation of kanamycin via porins is relevant for antibiotic potency. The values are discussed with respect to other antibiotics.
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Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Kanamicina
/
Escherichia coli
Idioma:
En
Revista:
ACS Infect Dis
Año:
2020
Tipo del documento:
Article
País de afiliación:
Alemania