Optimized Batrachochytrium dendrobatidis DNA extraction of swab samples results in imperfect detection particularly when infection intensities are low.

Brannelly, Laura A; Wetzel, Daniel P; West, Matt; Richards-Zawacki, Corinne L

Brannelly, Laura A; Wetzel, Daniel P; West, Matt; Richards-Zawacki, Corinne L.

Afiliación

Brannelly LA; One Health Research Group, Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Werribee, Victoria 3030, Australia.

Dis Aquat Organ ; 139: 233-243, 2020 Jun 04.

Article en En | MEDLINE | ID: mdl-32495749

Asunto(s)

Quitridiomicetos; Infecciones; Anfibios; Animales; ADN; Manejo de Especímenes

Palabras clave

Amphibian disease; Chelex resin; Chytridiomycosis; DNA extraction; Detection threshold; False negative; Pathogen detection; PrepMan Ultra; Qiagen DNeasy Blood and Tissue

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Quitridiomicetos / Infecciones Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Dis Aquat Organ Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA / MICROBIOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Australia

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