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A Preclinical Controlled Cortical Impact Model for Traumatic Hemorrhage Contusion and Neuroinflammation.
Lee, Hung-Fu; Chen, Chih Hung; Chang, Che-Feng.
Afiliación
  • Lee HF; Department of Neurosurgery, Cheng Hsin General Hospital.
  • Chen CH; Graduate Institute of Physiology, College of Medicine, National Taiwan University.
  • Chang CF; Graduate Institute of Physiology, College of Medicine, National Taiwan University; chefengchang@ntu.edu.tw.
J Vis Exp ; (160)2020 06 10.
Article en En | MEDLINE | ID: mdl-32597878
ABSTRACT
Cerebral contusion is a severe medical problem affecting millions of people worldwide each year. There is an urgent need to understand the pathophysiological mechanism and to develop effective therapeutic strategy for this devastating neurological disorder. Intraparenchymal hemorrhage and post-traumatic inflammatory response induced by initial physical impact can aggravate microglia/macrophage activation and neuroinflammation which subsequently worsen brain pathology. We provide here a controlled cortical impact (CCI) protocol that can reproduce experimental cortical contusion in mice by using a pneumatic impactor system to deliver mechanical force with controllable magnitude and velocity onto the dural surface. This preclinical model allows researchers to induce moderately severe focal cerebral contusion in mice and to investigate a wide range of post-traumatic pathological progressions including hemorrhage contusion, microglia/macrophage activation, iron toxicity, axonal injury, as well as short-term and long-term neurobehavioral deficits. The present protocol can be useful for exploring the long-term effects of and potential interventions for cerebral contusion.
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Corteza Cerebral / Contusiones / Lesiones Traumáticas del Encéfalo / Hemorragia / Inflamación Tipo de estudio: Guideline Límite: Animals Idioma: En Revista: J Vis Exp Año: 2020 Tipo del documento: Article

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Corteza Cerebral / Contusiones / Lesiones Traumáticas del Encéfalo / Hemorragia / Inflamación Tipo de estudio: Guideline Límite: Animals Idioma: En Revista: J Vis Exp Año: 2020 Tipo del documento: Article