Enhancement of glucaric acid production in Saccharomyces cerevisiae by expressing Vitreoscilla hemoglobin.
Biotechnol Lett
; 42(11): 2169-2178, 2020 Nov.
Article
en En
| MEDLINE
| ID: mdl-32691185
ABSTRACT
OBJECTIVE:
To enhance the glucaric acid (GA) production in Saccharomyces cerevisiae, the Vitreoscilla hemoglobin was employed to reinforce cellular oxygen supplement. Additionally, the pH-free fermentation strategy was engaged to lower the cost brought by base feeding during the acid-accumulated and long-period glucaric acid production.RESULTS:
Recombinant yeast Bga-4 was constructed harboring Vitreoscilla hemoglobin on the basis of previous Bga-3. Higher glucose uptake rate, growth rate, and ethanol reuse rate were achieved in Bga-4 in shake-flask fermentation than those in Bga-3. Furthermore, the fed-batch fermentation in a 5-L bioreactor was performed without pH control, resulting in a final glucaric acid titer of 6.38 g/L.CONCLUSIONS:
Both the GA titer and biomass were enhanced along with the efficiency of ethanol re-utilization in the presence of VHb. Moreover, the absence of base feeding for long-period fermentation reduced production cost, which is meaningful for industrial applications.Palabras clave
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Bases de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
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Proteínas Bacterianas
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Hemoglobinas Truncadas
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Ácido Glucárico
Idioma:
En
Revista:
Biotechnol Lett
Año:
2020
Tipo del documento:
Article