Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques.
Arch Virol
; 165(10): 2373-2377, 2020 Oct.
Article
en En
| MEDLINE
| ID: mdl-32761270
ABSTRACT
In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Neumonía Viral
/
ARN Viral
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Infecciones por Coronavirus
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Técnicas de Laboratorio Clínico
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Betacoronavirus
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
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Humans
Idioma:
En
Revista:
Arch Virol
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos