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Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques.
Carossino, Mariano; Ip, Hon S; Richt, Juergen A; Shultz, Kendra; Harper, Kimberly; Loynachan, Alan T; Del Piero, Fabio; Balasuriya, Udeni B R.
Afiliación
  • Carossino M; Louisiana Animal Disease Diagnostic Laboratory, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, River Rd, Room 1043, Baton Rouge, LA, 70803, USA.
  • Ip HS; United States Department of the Interior, U. S. Geological Survey, National Wildlife Health Center, 6006, Schroeder Road, Madison, WI, 53711, USA.
  • Richt JA; Department of Diagnostic Medicine/Pathobiology, Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD), Kansas State University, K-224B Mosier Hall, Manhattan, KS, 66506, USA.
  • Shultz K; Louisiana Animal Disease Diagnostic Laboratory, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, River Rd, Room 1043, Baton Rouge, LA, 70803, USA.
  • Harper K; Louisiana Animal Disease Diagnostic Laboratory, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, River Rd, Room 1043, Baton Rouge, LA, 70803, USA.
  • Loynachan AT; University of Kentucky Veterinary Diagnostic Laboratory, Department of Veterinary Science, University of Kentucky, Lexington, KY, 40511, USA.
  • Del Piero F; Louisiana Animal Disease Diagnostic Laboratory, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, River Rd, Room 1043, Baton Rouge, LA, 70803, USA.
  • Balasuriya UBR; Louisiana Animal Disease Diagnostic Laboratory, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, River Rd, Room 1043, Baton Rouge, LA, 70803, USA. balasuriya1@lsu.edu.
Arch Virol ; 165(10): 2373-2377, 2020 Oct.
Article en En | MEDLINE | ID: mdl-32761270
ABSTRACT
In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Neumonía Viral / ARN Viral / Infecciones por Coronavirus / Técnicas de Laboratorio Clínico / Betacoronavirus Tipo de estudio: Diagnostic_studies Límite: Animals / Humans Idioma: En Revista: Arch Virol Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Neumonía Viral / ARN Viral / Infecciones por Coronavirus / Técnicas de Laboratorio Clínico / Betacoronavirus Tipo de estudio: Diagnostic_studies Límite: Animals / Humans Idioma: En Revista: Arch Virol Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos