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Ketamine potentiates TRPV1 receptor signaling in the peripheral nociceptive pathways.
da Costa, Flavia Lage Pessoa; Pinto, Mauro Cunha Xavier; Santos, Duana Carvalho; Carobin, Natália Virtude; de Jesus, Itamar Couto Guedes; Ferreira, Luana Assis; Guatimosim, Silvia; Silva, Juliana Figueira; Castro Junior, Célio José.
Afiliación
  • da Costa FLP; Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Pinto MCX; Departamento de Farmacologia, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
  • Santos DC; Santa Casa de Belo Horizonte Ensino e Pesquisa, Belo Horizonte, Minas Gerais, Brazil.
  • Carobin NV; Santa Casa de Belo Horizonte Ensino e Pesquisa, Belo Horizonte, Minas Gerais, Brazil.
  • de Jesus ICG; Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
  • Ferreira LA; Santa Casa de Belo Horizonte Ensino e Pesquisa, Belo Horizonte, Minas Gerais, Brazil.
  • Guatimosim S; Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
  • Silva JF; Santa Casa de Belo Horizonte Ensino e Pesquisa, Belo Horizonte, Minas Gerais, Brazil.
  • Castro Junior CJ; Santa Casa de Belo Horizonte Ensino e Pesquisa, Belo Horizonte, Minas Gerais, Brazil. Electronic address: celiojunior@santacasabh.org.br.
Biochem Pharmacol ; 182: 114210, 2020 12.
Article en En | MEDLINE | ID: mdl-32882205
TRPV1 is a cation channel expressed in peripheral nociceptive pathways and its activation can trigger nociception signals to the brain. Ketamine is an intravenous anesthetic routinely used for anesthesia induction and with potent analgesic activity. Despite its proven depressant action on peripheral sensory pathways, the relationship between ketamine and TRPV1 receptors is still unclear. In this study, we evaluated the effect of ketamine injected peripherally in a rat model of spontaneous pain induced by capsaicin. We also investigated the effect of ketamine on Ca2+ transients in cultured dorsal root ganglia (DRG) neurons and HEK293 cells expressing the TRPV1 receptor (HEK-TRPV1 cells). Intraplantar administration of ketamine caused an unexpected increase in nocifensive behavior induced by capsaicin. Incubation of HEK-TRPV1 cells with 10 µM ketamine increased TRPV1 and PKCє phosphorylation. Ketamine potentiated capsaicin-induced Ca2+ transients in HEK-TRPV1 cells and DRG neurons. Ketamine also prevented TRPV1 receptor desensitization induced by successive applications of capsaicin. єV1-2, a PKCє inhibitor, reduced potentiation of capsaicin-induced Ca2+ transients by ketamine. Taken together, our data indicate that ketamine potentiates TRPV1 receptor sensitivity to capsaicin through a mechanism dependent on PKCє activity.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Transducción de Señal / Canales Catiónicos TRPV / Nocicepción / Ketamina Límite: Animals / Humans / Male Idioma: En Revista: Biochem Pharmacol Año: 2020 Tipo del documento: Article País de afiliación: Brasil

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Transducción de Señal / Canales Catiónicos TRPV / Nocicepción / Ketamina Límite: Animals / Humans / Male Idioma: En Revista: Biochem Pharmacol Año: 2020 Tipo del documento: Article País de afiliación: Brasil