A rapid dispersive liquid-liquid microextraction of antimicrobial onion organosulfur compounds in animal feed coupled to gas chromatography-mass spectrometry.
Anal Methods
; 12(21): 2668-2673, 2020 06 04.
Article
en En
| MEDLINE
| ID: mdl-32930297
ABSTRACT
A rapid analytical procedure is proposed for determining two antimicrobial onion organosulfur compounds, propyl disulfide (PDS) and propyl propane thiosulfonate (PTSO), in animal feed. The use of PTSO as a natural ingredient in animal feed is allowed due to its antimicrobial activity against pathogenic organisms. Two analytical methodologies using gas chromatography coupled to mass spectrometry (GC-MS) are compared. After the extraction of the compounds from animal feed with acetonitrile, dispersive solid phase extraction (DSPE) as a cleaning stage with C18, or dispersive liquid-liquid microextraction (DLLME), using 100 µL of CHCl3, was tried. Both the methods were validated using a pig feed sample and the best results were achieved by DLLME. This technique provided cleaner extracts, five-times greater linear ranges and lower detection limits than simple cleaning due to the enrichment factor achieved. The relative standard deviation decreased from 22% with DSPE to 13% with DLLME. The usefulness of the DLLME-GC-MS methodology was tested by analysing 10 different samples of chicken, calf, hen, cow and fish feed. The concentrations of PDS were in the 0.1-1.7 µg g-1 range and those of PTSO were between 0.09 and 2.1 µg g-1.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Microextracción en Fase Líquida
/
Antiinfecciosos
Límite:
Animals
Idioma:
En
Revista:
Anal Methods
Año:
2020
Tipo del documento:
Article
País de afiliación:
España