Prevalence Study of PD-L1 SP142 Assay in Metastatic Triple-negative Breast Cancer.
Appl Immunohistochem Mol Morphol
; 29(4): 258-264, 2021 04 01.
Article
en En
| MEDLINE
| ID: mdl-33030848
ABSTRACT
Metastatic triple-negative breast cancer (mTNBC) is the most aggressive breast cancer subtype. Programmed death ligand 1 (PD-L1) on immune cells (IC) using the VENTANA SP142 assay is linked to improved clinical outcome in atezolizumab plus nab-paclitaxel-treated patients with mTNBC in the IMpassion130 study. The goal of the current study was to evaluate prevalence of VENTANA SP142 PD-L1 assay by anatomic location in 670 histologically confirmed TNBC cases from subjects with metastatic disease screened for the phase 1 study PCD4989g (NCT01375842). PD-L1 immunohistochemistry was centrally tested on tumor cells (TC) and on tumor infiltrating IC, following manufacturer's instructions. At a 1% cutoff, tumor PD-L1 was more prevalent in IC than TC 46% were PD-L1 IC+/TC-, 3% were PD-L1 IC-/TC+, and 10% were PD-L1 IC+/TC+. PD-L1 IC and TC immunostaining correlated with CD274 RNA expression, as assessed by fluidigm. Analyses of anatomic locations suggest that prevalence of PD-L1 IC+ was highest in lymph nodes (65.0%), lowest in liver metastases (26.9%), while breast tissue was intermediate (57.1%). Matched paired samples from the same subject collected synchronously or asynchronously showed a PD-L1 IC status agreement of 80% (8/10) and 75% (15/20), respectively. Our results suggest that the anatomic location of metastases and time of collection may influence the detection of PD-L1.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Antígeno B7-H1
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Neoplasias de la Mama Triple Negativas
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Proteínas de Neoplasias
Tipo de estudio:
Clinical_trials
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Prevalence_studies
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Risk_factors_studies
Límite:
Female
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Humans
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Middle aged
Idioma:
En
Revista:
Appl Immunohistochem Mol Morphol
Asunto de la revista:
BIOLOGIA MOLECULAR
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HISTOCITOQUIMICA
Año:
2021
Tipo del documento:
Article
País de afiliación:
Canadá