Improvement of early developmental competence of postovulatory-aged oocytes using metaphase II spindle injection in mice.
Reprod Med Biol
; 19(4): 357-364, 2020 Oct.
Article
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| MEDLINE
| ID: mdl-33071637
ABSTRACT
PURPOSE:
Assisted reproductive technology (ART) is a widely applied fertility treatment. However, the developmental competence of aged oocytes from women of a late reproductive age is seriously reduced and the aged oocytes often fail in fertilization even when ART is used. To resolve this problem, we examined usefulness of a new method "the metaphase II spindle transfer (MESI)" as ART using mouse oocytes.METHODS:
This work was composed of two experiments. First, 24 hours after collection, embryos from oocytes (1-day-old oocytes, called postovulatory-aged oocytes), were observed, after intracytoplasmic sperm injection (ICSI), and it was found that they were not able to reach the blastocyst stage. Next, the metaphase II chromosome-spindle complexes from 1-day-old oocytes were injected into cytoplasts from oocytes just collected, using piezo pulses to generate reconstructed oocytes. This procedure was named metaphase II spindle injection (MESI).RESULTS:
After ICSI, embryos from the reconstructed oocytes (32/105), which contained the genes of 1-day-old oocytes, were able to develop into the blastocyst stage. The fragmentation rate after ICSI was 28.6%. Thus, the developmental competence of 1-day-old oocytes was improved by MESI.CONCLUSIONS:
The MESI method has the potential to improve the success rate of infertility treatments for women of a late reproductive age.
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MEDLINE
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Reprod Med Biol
Año:
2020
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Article