Glycocyamine functionalized magnetic layered double hydroxides with multiple affinity sites for trace phosphopeptides enrichment.
Anal Chim Acta
; 1136: 25-33, 2020 Nov 01.
Article
en En
| MEDLINE
| ID: mdl-33081946
ABSTRACT
Efficient enrichment and identification of phosphopeptides are of great significance in biological applications. Glycocyamine functionalized magnetic layered double hydroxides (Fe3O4@LDH@NH2-GAA) was fabricated through an easy process. The magnetic composite possessed high surface area, good biocompatibility, and fast magnetic response. Fe3O4@LDH@NH2-GAA, combining not only metal ions (Cu2+ and Ga3+) in LDH but also functional guanidyl groups in glycocyamine, offered multiple affinity sites for phosphopeptides enrichment. With these favorable characters, it exhibited high selectivity (ß-casein bovine serum albumin = 15000), low detection limit (0.1 fmol), satisfactory enrichment recovery (94.5%), high adsorption capacity (82.4 mg g-1), and good repetitiveness. Moreover, the efficient enrichment of phosphopeptides by Fe3O4@LDH@NH2-GAA from nonfat milk, human saliva, serum, and A549 cell lysates further confirmed its great potential for trace biological detection and proteomic analysis.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Fosfopéptidos
/
Proteómica
Límite:
Humans
Idioma:
En
Revista:
Anal Chim Acta
Año:
2020
Tipo del documento:
Article